Journal of Biotechnology & Biomaterials
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In Association with
Exploration and Purification of Bioactive compound from seaweeds against human bacterial pathogen
2nd World Congress on Bio Summit & Molecular Biology Expo
Sahaya Sukeetha D and John Antony B
Research Scholar, Bharathidasan University, Trichy, India MPEDA, Nagapattinam, India
ScientificTracks Abstracts: J Biotechnol Biomater
DOI:
Abstract
Treatment of Infectious diseases by the use of commercially available drugs are becoming certain limitations due to changing patterns of resistance in pathogens and causing side effects. These limitations demand for improved pharmacokinetic properties which necessitates continued research for the search of new novel drugs. Marine organisms are rich source of structurally novel and biologically active metabolites. The cell extracts and active constituents of various algae and seaweeds have been shown to have antibacterial activity against gram positive and gram negative bacteria. Hence the crude extracts from the seaweeds Amphiroa foliacea, Chactomorpho tortuosa, Caulerpa scalpelliformis and Sargassum sp were tested for their resistance against multidrug resistance pathogens such as Staphylococcus aureus, Klebsella sp, Proteus sp. The extracts were obtained with the solvents methanol, chloroform, ethyl acetate and hexane. A highest zone of inhibition was observed in the hexane extract of Amphiroa foliacea and ethyl acetate extract of Sargassam sp against Proteus sp, Staphylococcus aureus respectively. An inhibition zone of 8mm was observed in ethyl acetate extract of Sargassam sp against Staphylococcus aureus. Further the extract of Sargassum sp was purified using silica column chromatography. Single compound fractions were separated and each fraction was screened for antibacterial activity against Staphylococcus aureus. F4 fraction possessed antibacterial activity of 7mm which is similar to crude extract. Further the F4 fraction is subjected NMR analysis. Ethyl acetate fraction was found to be posses �?卤-hydroxy stearic acid.Biography
D. Sahaya Sukeetha M.Sc., M.Phil., (Ph.D) is an graduate from Bharathidasan University. Presently she is working as a scientific officer in the department of molecular biology, Inbiotics, Nagercoil. She had worked as an Assistant professor for 7 years and handled biotechnology classes for both under graduate and post graduate students. Also she had guided 12 project post graduate students. In her academic career she had published 5 research papers and attended various conferences.
Email: sukeethabt@gmail.com
