ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
91勛圖
Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 91勛圖 Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
91勛圖 Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Citations : 3330
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- ResearchBible
- China National Knowledge Infrastructure (CNKI)
- Access to Global Online Research in Agriculture (AGORA)
- Electronic Journals Library
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- ICMJE
Useful Links
Recommended Journals
Related Subjects
Share This Page
In Association with
Development of glyphosate-tolerant transgenic potato plants harboring the G2aroA gene
Annual Biotechnology Congress
Arfan Ali
Four Brothers Grouplahore, Pakistan
Posters & Accepted Abstracts: J Biotechnol Biomater
DOI:
Abstract
Glyphosate weed control is a very effective strategy to minimize cost and improve economic outcomes of world and Pakistan agriculture production. Development of glyphosate -resistant potato holds great promise. A new G2-aoraA gene from Pseudomonas florescence which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was transformed by using an Agrobacterium-mediated transformation into potato cultivate AGB-Red. Transgenic potato plants were generated vis node tissue culture method using kanamycin selection. 10 regenerated potato plants were obtained and allowed to grow normally in pots under normal conditions. The polymerase chain reaction (PCR), southern blotting and western blotting analysis confirmed that the target gene was integrated and expressed effectively into potato chromosomes at the very potential level. The glyphosate tolerance assay showed that transgenic potato had a high resistance level to glyphosate. Furthermore, potato plants treated with 50.0 mili mole of glyphosate could grow slowly and can develop tubers. It was concluded that transgenic potato may be used for cotton breeding research of glyphosate -tolerant potato.Biography
Arfan Ali is from Centre for Excellence in Molecular Biology, University of the Punjab and Biotechnology Lab Four Brothers Group, Lahore, Pakistan.
E-mail: arfan.ali@cemb.edu.pk
