91勛圖

ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
91勛圖

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Bioconjugation studies of GO/Fe3O4 nanocomposites hollow/porous magnetite

4th Annual Conference and Expo on Biomaterials

Amodini Mishra, Bijoy Kumar Kuanr and Tanuja Mohanty

Jawaharlal Nehru University, India

Posters & Accepted Abstracts: J Biotechnol Biomater

DOI:

Abstract
In this paper, we have synthesized reduced graphene oxide/magnetite (rGO-Fe3O4) nanocomposites by chemical coprecipitation method for a comparative Raman spectroscopic study. The nanocomposites along with its pristine GO and Fe3O4 counterpart were modified covalently with a fluorescently labeled protein. The modification was confirmed using confocal fluorescence microscopy. The GO, Fe3O4 and rGO-Fe3O4 samples were characterized by different spectroscopic and microscopic techniques before and after protein conjugation. A significant enhancement in Raman peaks obtained in case of protein modified rGO-Fe3O4 nanocomposites compared to pristine GO and Fe3O4explains active Surface Enhancement Raman Spectroscopy (SERS) effect. An unusual phenomenon of GO to rGO conversion and vice-versa was noted as a result of covalent protein attachment.
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