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Abstract

Electrospinning has been a popular technique to obtain nanofibrous membranes from synthetic and natural polymer sources. In contrary to synthetic polymers, the production capabilities of natural polymer membranes failed at some extend by means of electrospinnability and efficiency also biocompatibility. In this paper, we introduced an enzyme immobilization platform from a natural polymer membrane. For this purpose, a model protein bovine serum albumin (BSA) was chosen mainly for enhanced supporting property. To procure electrospinnable solution of BSA, beta-mercaptoethanol (�²-ME) was used to induce tertiary structure and low ratio (1.5:1.0 TFE:PBS (pH: 7.4)) of 2,2,2-triflouroethanol (TFE) was added as a stabilizing agent, respectively. The electrospun membranes were activated with RF plasma treatment by employing ethylenediamine (EDA) as a precursor to incorporate amino (-NH2) groups on the surface. Those surfaces were cross-linked with glutaraldehyde aqueous solutions at concentrations between 0.01 and 5% wt. which followed by the covalent attachment of glucose oxidase (GOD). The performance of enzyme immobilized membranes was tested by employing amperometric measurements against various glucose concentrations in terms of response time, enzymatic activity and linearity. The effects of plasma parameters and crosslinking conditions on the performance of protein membrane based enzyme electrode were also studied.

Biography

Email: gkabay@etu.edu.tr